Recombinant Human GUCY2C protein, Fc-tagged

• Cat.No.: GUCY2C-669H
• Product Overview: Recombinant Human GUCY2C protein (Ser24-Gln430) was fused to human IgG1 Fc tag at the C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Predicted N Terminal: Ser24
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ser24-Gln430
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GUCY2C
• Official Symbol: GUCY2C
• Synonyms: GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C
• Gene ID: 2984
• mRNA Refseq: NM_004963.4
• Protein Refseq: NP_004954.2
• MIM: 601330
• UniProt ID: P25092