Recombinant Human GUCY2C protein, Fc-tagged GUCY2C-669H

Recombinant Human GUCY2C protein, Fc-tagged

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Recombinant Human GUCY2C protein, Fc-tagged

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Cat.No. : GUCY2C-669H
Product Overview : Recombinant Human GUCY2C protein (Ser24-Gln430) was fused to human IgG1 Fc tag at the C-terminus and expressed in human 293 cells (HEK293).
Description : GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Ser24
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Ser24-Gln430
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of GUCY2C-669H :

Human GUCY2C, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of GUCY2C-669H :

Immobilized Human GUCY2C, Fc Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Bioactivity-FACS of GUCY2C-669H :

2e5 of anti-GUCY2C CAR-293 cells were stained with 100 µL of 1 µg/mL Human GUCY2C, Fc Tag and negative control protein respectively, washed and then followed by PE anti-human IgG Fc and analyzed with FACS (Routinely tested).

Gene Name : GUCY2C
Official Symbol : GUCY2C
Synonyms : GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C
Gene ID : 2984
mRNA Refseq : NM_004963.4
Protein Refseq : NP_004954.2
MIM : 601330
UniProt ID : P25092

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