Recombinant Human HBB protein, His & GST-tagged

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Recombinant Human HBB protein, His & GST-tagged

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Cat.No. : HBB-7865H
Product Overview : Recombinant Human HBB aa. (Met1~His147 (Accession # P68871)) fused with N-terminal His & GST tag was produced in E. coli cells.
Description : The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Source : E. coli
Species : Human
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : 46kDa as determined by SDS-PAGE reducing conditions.
Protein length : Met1~His147 (Accession # P68871)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 6.6.
Applications : SDS-PAGE; WB; ELISA; IP
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage Buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Reconstitution : Reconstitute in ddH2O.
SDS-PAGE :

Gene Name : HBB hemoglobin subunit beta [ Homo sapiens (human) ]
Official Symbol : HBB
Synonyms : HBB; hemoglobin subunit beta; CD113t-C; beta-globin; beta globin chain; hemoglobin, beta
Gene ID : 3043
mRNA Refseq : NM_000518.4
Protein Refseq : NP_000509.1
UniProt ID : P68871

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