Description : |
Hemoglobin subunit theta-1, also known as HBQ1, belongs to the Hemoglobin family. Hemoglobin (Hgb) is a 66.7 kDa protein coupled to four iron-binding, methenelinked tetrapyrrole rings (heme). The globin portion of Hgb consists of two alpha chains and two beta chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between alpha and beta chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the alpha1-beta2 cleavage plane. |
Source : |
E. coli |
Species : |
Human |
Tag : |
His |
Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
Molecular Mass : |
17.9 kDa (165aa), confirmed by MALDI-TOF |
Protein length : |
1-142aa |
AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MGSMALSAED RALVRALWKK LGSNVGVYTT EALERTFLAF PATKTYFSHL DLSPGSSQVR AHGQKVADAL SLAVERLDDL PHALSALSHL HACQLRVDPA SFQLLGHCLL VTLARHYPGD FSPALQASLD KFLSHVISAL VSEYR |
Purity : |
>90% by SDS - PAGE |
Applications : |
SDS-PAGE |
Storage : |
Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration : |
1 mg/ml (determined by Bradford assay) |