Recombinant Human HBQ1, His-tagged

• Cat.No.: HBQ1-15874H
• Product Overview: Recombinant human HBQ1 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-142aa
• Description: Hemoglobin subunit theta-1, also known as HBQ1, belongs to the Hemoglobin family. Hemoglobin (Hgb) is a 66.7 kDa protein coupled to four iron-binding, methenelinked tetrapyrrole rings (heme). The globin portion of Hgb consists of two alpha chains and two beta chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between alpha and beta chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the alpha1-beta2 cleavage plane.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 17.9 kDa (165aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMALSAED RALVRALWKK LGSNVGVYTT EALERTFLAF PATKTYFSHL DLSPGSSQVR AHGQKVADAL SLAVERLDDL PHALSALSHL HACQLRVDPA SFQLLGHCLL VTLARHYPGD FSPALQASLD KFLSHVISAL VSEYR
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HBQ1 hemoglobin, theta 1 [ Homo sapiens (human) ]
• Official Symbol: HBQ1
• Synonyms: HBQ1; hemoglobin, theta 1; hemoglobin subunit theta-1; theta-1-globin; hemoglobin theta-1 chain
• Gene ID: 3049
• mRNA Refseq: NM_005331
• Protein Refseq: NP_005322
• MIM: 142240