Active Recombinant Full Length Human HDAC11 Protein, Tag Free

• Cat.No.: HDAC11-92H
• Product Overview: Recombinant full length human HDAC11 (residues 1-347), expressed without tag in an insect cell/baculovirus system.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: 1-347 aa
• Description: This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.
• Form: 50 mM Tris/HCl, pH 8.0, 500 mM NaCl, 10% glycerol (v/v)
• Bio-activity: HDAC11 displays deacetylase activity with the fluorigenic substrate Boc-Lys(TFA)-AMC. Reactions were performed at 37 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/mL BSA. Reactions were stopped and fluorescence signals developed by addition of an equal volume of "Developer" (16 mg/mL trypsin/2 μM trichostatin A in the same buffer). Fluorescence (Ex. 355 nm/Em. 460 nm) was read every 5 min., until signal stabilized, indicating that trypsin cleavage of the deacetylated peptide (Boc-Lys(TFA)-AMC), to release the AMC fluorophor was complete (< 2 hrs.)
• Molecular Mass: 42.2 kDa
• Purity: > 75% by SDS-PAGE
• Storage: At -70 centigrade. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (< 5 μL) or storage of diluted enzyme is not recommended.

Gene Information

• Gene Name: HDAC11 histone deacetylase 11 [ Homo sapiens (human) ]
• Official Symbol: HDAC11
• Synonyms: HDAC11; histone deacetylase 11; HD11; histone deacetylase 11; EC 3.5.1.98
• Gene ID: 79885
• mRNA Refseq: NM_024827
• Protein Refseq: NP_079103
• MIM: 607226
• UniProt ID: Q96DB2