Recombinant Human Heat Shock Protein Beta-1, His tagged

• Cat.No.: HSPB1-1825H
• Product Overview: Recombinant human HSP27 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by usin.

Specification

• Cat. No.: HSPB1-1825H
• Description: Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) contining 1mM DTT 10% glycerol
• Molecular Weight: 24.9 kDa (225aa)
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method )
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: MAPK signaling pathway; VEGF signaling pathway

Gene Information

• Gene Name: HSPB1 heat shock 27kDa protein 1 [ Homo sapiens ]
• Synonyms: HSPB1; CMT2F; DKFZp586P1322; HS.76067; HSP28; Hsp25; HMN2B; HSP27; SRP27; HS.76067; DKFZp586P1322; heat shock protein beta-1; stress-responsive protein 27; estrogen-regulated 24 kDa protein.; OTTHUMP00000024846; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kDa protein 1.
• Gene ID: 3315
• mRNA Refseq: NM_001540
• Protein Refseq: NP_001531
• MIM: 602195
• UniProt ID: P04792
• Chromosome Location: 7q11.23
• Function: identical protein binding; ubiquitin binding