Recombinant Human HERC4, GST-tagged

• Cat.No.: HERC4-178H
• Product Overview: Recombinant human HERC4 (642-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: HERC4 or HECT and RLD domain containing E3 ubiquitin protein ligase 4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD domain is predicted to act as a guanine nucleotide exchange factor for small G proteins. HERC4 is highly expressed in adult and fetal brain and in all specific brain regions examined.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~71 kDa
• Purity: >80% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: HERC4 HECT and RLD domain containing E3 ubiquitin protein ligase 4 [ Homo sapiens ]
• Official Symbol: HERC4
• Synonyms: HERC4; HECT and RLD domain containing E3 ubiquitin protein ligase 4; hect domain and RLD 4; probable E3 ubiquitin-protein ligase HERC4; DKFZP564G092; KIAA1593; HECT domain and RCC1-like domain-containing protein 4; DKFZp564G092
• Gene ID: 26091
• mRNA Refseq: NM_015601
• Protein Refseq: NP_056416
• MIM: 609248
• UniProt ID: Q5GLZ8
• Chromosome Location: 10
• Pathway: Ubiquitin mediated proteolysis, organism-specific biosystem; Ubiquitin mediated proteolysis, conserved biosystem
• Function: acid-amino acid ligase activity; ligase activity; ubiquitin-protein ligase activity