Recombinant Human HGF, 495-728aa, His-tagged

• Cat.No.: HGF-226H
• Product Overview: Recombinant Human HGF encoding the human HGF beta chain (Val495–Ser728) was recombined with his tag at the N-Terminus. That was expressed inE.Coli,and purified by Ni-NTA column. MW=26KD.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 495-728 a.a.
• Description: Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
• Sequence: Human HGF beta chain (Val⁴⁹⁵–Ser⁷²⁸)495 VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS 728.
• Formulation: Lyophilized 100 μg human HGF beta in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
• Application: Cell biology, ELISA.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
• Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
• Synonyms: HGF; hepatocyte growth factor (hepapoietin A; scatter factor); SF; HGFB; HPTA; F-TCF; DFNB39; scatter factor; hepatopoietin A; OTTHUMP00000206710; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; DFNB39; deafness, autosomal recessive 39; fibroblast-derived tumor cytotoxic factor; scatter factor; Hepatocyte growth factor; SF; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chainen
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210
• Chromosome Location: 7q21.1
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Melanoma; Pathways in cancer; Renal cell carcinoma; Hemostasis
• Function: catalytic activity; growth factor activity; protein binding; protein heterodimerization activity; serine-type endopeptidase activity