Recombinant Human High-mobility Group Box 1, His-tagged

• Cat.No.: HMGB1-532H
• Product Overview: Recombinant human HMGB1 was expressed inHigh Five insect cellsand purified by conventional chromatography techniques. MW = 25kDa (223aa).

Specification

• Species: Human
• Source: Hi-5 Insect Cells
• Tag: His
• Description: High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.
• Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5 mM DTT, 1 mM EDTA, 10% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HMGB1 high-mobility group box 1 [ Homo sapiens ]
• Synonyms: HMGB1; DKFZp686A04236; HMG1; HMG3; SBP-1; HMG3; high-mobility group box 1; Amphoterin; OTTHUMP00000018199; OTTHUMP00000190860; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1; Amphoterin, High-Mobility Group Box 1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 601912
• UniProt ID: P09429
• Chromosome Location: 13q12
• Function: DNA bending activity; DNA binding; transcription factor binding