Recombinant Human HIPK2 Protein, GST-tagged

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Recombinant Human HIPK2 Protein, GST-tagged

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Cat.No. : HIPK2-4761H
Product Overview : Human HIPK2 (Q9H2X6, 1 a.a. - 1198 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.[supplied by OMIM
Source : Insect Cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
Substrate: DYRKtide-F.
ATP: 100 μM.
Molecular Mass : 158 kDa
Purity : 76 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : HIPK2 homeodomain interacting protein kinase 2 [ Homo sapiens ]
Official Symbol : HIPK2
Synonyms : HIPK2; homeodomain interacting protein kinase 2; homeodomain-interacting protein kinase 2; hHIPk2; PRO0593; FLJ23711; DKFZp686K02111;
Gene ID : 28996
mRNA Refseq : NM_001113239
Protein Refseq : NP_001106710
MIM : 606868
UniProt ID : Q9H2X6

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