Recombinant Human Histamine N-Methyltransferase, His-tagged

• Cat.No.: HNMT-1704H
• Product Overview: Recombinant human HNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Cat. No.: HNMT-1704H
• Description: Histamine N-methyltransferase (HNMT) is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT inactivates histamine by N-methylation. Histamine is involved in regulation and modulation of immune response through the stimulation of four distinct subtypes of receptors, H1, H2, H3, and H4, present on the target cells. Histamine is inactivated by the histamine-metabolizing enzyme histamine N-methyltransferase (HNMT) in bronchus, kidney, and the central nervous system. It plays an important role in degrading histamine and in regulating the airway response to histamine.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: 1-292 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSS
• Molecular Mass: 37 kDa (328 aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HNMT histamine N-methyltransferase [ Homo sapiens ]
• Official Symbol: HNMT
• Synonyms: EC 2.1.1.8; HMT; HNMT-S1; HNMT-S2; histamine N-methyltransferase; HNMT; 5` and 3` partial
• Gene ID: 3176
• mRNA Refseq: NM_001024074
• Protein Refseq: NP_001019245
• MIM: 605238
• UniProt ID: P50135
• Chromosome Location: 2q22.1
• Pathway: Histidine metabolism
• Function: histamine N-methyltransferase activity; methyltransferase activity; transferase activity