Recombinant Human Histamine N-Methyltransferase, His-tagged

• Cat.No.: HNMT-1704H
• Product Overview: Recombinant human HNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Histamine N-methyltransferase (HNMT) is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT inactivates histamine by N-methylation. Histamine is involved in regulation and modulation of immune response through the stimulation of four distinct subtypes of receptors, H1, H2, H3, and H4, present on the target cells. Histamine is inactivated by the histamine-metabolizing enzyme histamine N-methyltransferase (HNMT) in bronchus, kidney, and the central nervous system. It plays an important role in degrading histamine and in regulating the airway response to histamine.
• Concentration: 1 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: 1-292 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSS
• Molecular Mass: 37 kDa (328 aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: HNMT histamine N-methyltransferase [ Homo sapiens ]
• Official Symbol: HNMT
• Synonyms: EC 2.1.1.8; HMT; HNMT-S1; HNMT-S2; histamine N-methyltransferase; HNMT; 5` and 3` partial
• Gene ID: 3176
• mRNA Refseq: NM_001024074
• Protein Refseq: NP_001019245
• MIM: 605238
• UniProt ID: P50135
• Chromosome Location: 2q22.1
• Pathway: Histidine metabolism
• Function: histamine N-methyltransferase activity; methyltransferase activity; transferase activity

Citation

TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1

Journal: Science Advances    PubMed ID: 27757426    Data: 2016/4/1

Authors: Mohammad B. Hossain, Rehnuma Shifat, Candelaria Gomez-Manzano

Article Snippet:In vitro kinase assays were conducted with 200 ng of purified glutathione S -transferase (GST)–tagged TIE2 active protein (SignalChem, no. T04-11G) or the His-tagged TIE2 with deleted kinase domain (Creative BioMart, no. TEK-153H) in kinase buffer [25 mM MOPS (pH 7.2), 12.5 mM β-glycerol phosphate, 20 mM MgCl 2 , 12.5 mM MnCl2, 2 mM EDTA (pH 8.0), 5 mM EGTA (pH 7.0), and 0.25 mM DTT] with the addition of 20 μM adenosine triphosphate (ATP) for cold reactions, and 10 μM ATP mixed with 10 μCi of [γ- 32 P]ATP for radioactive reactions.. The substrates were added to the reaction mixture and incubated at 37°C for 30 min.The substrates were added to the reaction mixture and incubated at 37°C for 30 min.

Structure and Interactions of a Dimeric Variant of sHIP, a Novel Virulence Determinant of Streptococcus pyogenes

Journal: Frontiers in Microbiology    PubMed ID: 26903974    Data: 2016/2/5

Authors: Carl Diehl, Magdalena Wisniewska, Mats Wikstr?m

Article Snippet:Subsequently, the bacteria were diluted to a concentration of 2 × 10 6 cfu/ml in 10 mM MES buffer, pH 5.5, containing 5 mM glucose.Subsequently, the bacteria were diluted to a concentration of 2 × 10 6 cfu/ml in 10 mM MES buffer, pH 5.5, containing 5 mM glucose.. Fifty microliters of the bacterial solution was incubated with recombinant His-tagged HRG (Creative BioMart) at a concentration of 0.45 μM together with various concentrations of protein sHIPwt or protein sHIPqp for 40 min at 37°C.. Serial dilutions of the incubation mixtures were plated on TH agar, plates were incubated over night at 37°C and the number of cfu's were determined.Serial dilutions of the incubation mixtures were plated on TH agar, plates were incubated over night at 37°C and the number of cfu's were determined.

C1q-tumour necrosis factor-related protein 8 (CTRP8) is a novel interaction partner of relaxin receptor RXFP1 in human brain cancer cells.

Journal: The Journal of pathology    PubMed ID: 24014093    Data: 2014/4/14

Authors: Aleksandra Glogowska, Usakorn Kunanuvat, Thomas Klonisch

Article Snippet:Next, we employed co-immunoprecipitation (IP) studies to study the interaction between RXFP1 and CTRP8 in HEK-RXFP1-HA stable transfectants.Next, we employed co-immunoprecipitation (IP) studies to study the interaction between RXFP1 and CTRP8 in HEK-RXFP1-HA stable transfectants.. Upon incubation of HEK-RXFP1 with recombinant His-tagged CTRP8 (Creative BioMart), HAtargeted co-IP and Western blot analysis revealed the successful pull down of both HA-RXFP1 and CTRP8-His (Fig. 12).. Similar results were obtained when HEK293T-RXFP1-HA transfectants were transiently transfected with a construct encoding human FLAG-tagged CTRP8 followed by HA-targeted immunoprecipitation and Western blot detection of HA and FLAG epitopes (data not shown).Similar results were obtained when HEK293T-RXFP1-HA transfectants were transiently transfected with a construct encoding human FLAG-tagged CTRP8 followed by HA-targeted immunoprecipitation and Western blot detection of HA and FLAG epitopes (data not shown).