Recombinant Human Histidine Ammonia-Lyase

• Cat.No.: HAL-2513H
• Product Overview: Recombinant Human Histidine Ammonia-Lyase protein, was expressed in Human HEK293 cells and purified by SDS-PAGE and Coomassie blue staining

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Histidine ammonia-lyase (or histidase, or histidinase) is an enzyme that in humans is encoded by the HAL gene. Histidase converts histidine into ammonia and urocanic acid. Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid.
• Preparation: HEK293T cells were transiently transfected with MegaTran Transfection Reagent and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit.
• Molecular Weight: 72.5 kDa
• Purity: > 80% as determinedby SDS-PAGE and Coomassie blue staining
• Concentration: > 50 ug/ml as determined by BCA
• Buffer: 10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions

Gene Information

• Gene Name: HAL, histidine ammonia-lyase [Homo sapiens]
• Official Symbol: HAL
• Synonyms: HAL; histidine ammonia-lyase; HIS; HSTD; histidine ammonia-lyase; histidase
• Gene ID: 3034
• mRNA Refseq: NM_002108
• Protein Refseq: NP_002099
• MIM: 609457
• UniProt ID: P42235
• Chromosome Location: 12q22-q24.1
• Pathway: Histidine catabolism; Histidine metabolism; Metabolic pathways; Metabolism of amino acids and derivatives; Nitrogen metabolism; histidine degradation III; histidine degradation VI
• Function: histidine ammonia-lyase activity; lyase activity