Recombinant Human Histidine Ammonia-Lyase
Cat.No. : | HAL-2513H |
Product Overview : | Recombinant Human Histidine Ammonia-Lyase protein, was expressed in Human HEK293 cells and purified by SDS-PAGE and Coomassie blue staining |
- Specification
- Gene Information
- Related Products
Cat. No. : | HAL-2513H |
Description : | Histidine ammonia-lyase (or histidase, or histidinase) is an enzyme that in humans is encoded by the HAL gene. Histidase converts histidine into ammonia and urocanic acid. Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. |
Source : | Human HEK293 cells |
Preparation : | HEK293T cells were transiently transfected with MegaTran Transfection Reagent and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit. |
Molecular Weight : | 72.5 kDa |
Purity : | > 80% as determinedby SDS-PAGE and Coomassie blue staining |
Concentration : | > 50 ug/ml as determined by BCA |
Buffer : | 10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3 |
Storage : | Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions |
Gene Name : | HAL, histidine ammonia-lyase [Homo sapiens] |
Official Symbol : | HAL |
Synonyms : | HAL; histidine ammonia-lyase; HIS; HSTD; histidine ammonia-lyase; histidase |
Gene ID : | 3034 |
mRNA Refseq : | NM_002108 |
Protein Refseq : | NP_002099 |
MIM : | 609457 |
UniProt ID : | P42235 |
Chromosome Location : | 12q22-q24.1 |
Pathway : | Histidine catabolism; Histidine metabolism; Metabolic pathways; Metabolism of amino acids and derivatives; Nitrogen metabolism; histidine degradation III; histidine degradation VI |
Function : | histidine ammonia-lyase activity; lyase activity |
Products Types
◆ Recombinant Protein | ||
Hal-1094M | Recombinant Mouse Hal Protein, MYC/DDK-tagged | +Inquiry |
HAL-4563H | Recombinant Human HAL Protein, GST-tagged | +Inquiry |
HAL-831H | Recombinant Human HAL Protein, His-tagged | +Inquiry |
HAL-830H | Recombinant Human HAL Protein, MYC/DDK-tagged | +Inquiry |
HAL-2439R | Recombinant Rat HAL Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
HAL-5641HCL | Recombinant Human HAL 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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