Recombinant Human HLA-A*02:01 & B2M & GP100 (YLEPGPVTA) Protein, Biotinylated

• Cat.No.: HLA-A*02:01 & B2M & GP100-4619H
• Product Overview: Biotinylated Recombinant Human (YLEPGPVTA) Protein(Gly 25 - Thr 305 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & YLEPGPVTA peptide) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Molecular Mass: Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Complex Protein is produced by co-expression of HLA and B2M loaded with GP100 peptide.This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 36.0 kDa and 13.7 kDa. The protein migrates as 36-40 kDa, 17 kDa and 15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gly 25 - Thr 305 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & YLEPGPVTA peptide
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A
• Synonyms: HLA-A; major histocompatibility complex, class I, A; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; HLAA; FLJ26655
• Gene ID: 3105
• mRNA Refseq: NM_001242758
• Protein Refseq: NP_001229687
• UniProt ID: P01891