Recombinant Human HPX protein(Met 1-His 462), His-tagged

• Cat.No.: HPX-754H
• Product Overview: Recombinant Human HPX protein(NP_000604.1) (Met 1-His 462) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-462 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant human Hemopexin binds > 10 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin.
• Molecular Mass: The secreted recombinant human HPX comprises 450 amino acids with a predicted molecular mass of 50.7 kDa. As a result of glycosylation, rhHPX migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HPX hemopexin [ Homo sapiens ]
• Official Symbol: HPX
• Synonyms: HPX; hemopexin; beta-1B-glycoprotein; HX; FLJ56652
• Gene ID: 3263
• mRNA Refseq: NM_000613
• Protein Refseq: NP_000604
• MIM: 142290
• UniProt ID: P02790