Recombinant Human HSD17B3 protein, His-tagged HSD17B3-1407H

Recombinant Human HSD17B3 protein, His-tagged


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Recombinant Human HSD17B3 protein, His-tagged

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Cat.No. : HSD17B3-1407H
Product Overview : Recombinant Human HSD17B3 aa. (Met1~Arg310 fused with N-terminal His tag was produced in E. coli cells.
Description : This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 33kDa as determined by SDS-PAGE reducing conditions.
Protein length : Met1~Arg310
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >90%
Characteristic : The isoelectric point is 8.9.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Name : HSD17B3 hydroxysteroid 17-beta dehydrogenase 3 [ Homo sapiens (human) ]
Official Symbol : HSD17B3
Synonyms : HSD17B3; hydroxysteroid 17-beta dehydrogenase 3; EDH17B3; SDR12C2; testosterone 17-beta-dehydrogenase 3; 17-beta-HSD 3; 17-beta-HSD3; 17-beta-hydroxysteroid dehydrogenase type 3; hydroxysteroid dehydrogenase 3; short chain dehydrogenase/reductase family 12C member 2; testicular 17-beta-hydroxysteroid dehydrogenase
Gene ID : 3293
mRNA Refseq : NM_000197.1
Protein Refseq : NP_000188.1
UniProt ID : P37058

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