| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
1-310 aa |
| Form : |
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) belongs to the HSD17B family with NAD(P)H/NAD(P) -dependent oxidoreductase activity that catalyzes the interconversion between 17-ketosteroids and 17-hydroxysteroids to maintain the balance between less potent (17-keto) and more potent (17β-hydroxy) forms of estrogens and androgens. This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5a2 ) is a enzyme which plays a central role in sexual differentiation and androgen physiology. A functional ELISA assay was conducted to detect the interaction of recombinant human HSD17b3 and recombinant human SRD5a2. Briefly, HSD17b3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SRD5a2-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HSD17b3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human HSD17b3 and recombinant human SRD5a2 was shown, the EC50 for this effect is 27.6 ng/mL. |
| Molecular Mass : |
38.2kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
