Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Protein Length : |
Met1~Asp641 |
Description : |
This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. |
Form : |
Freeze-dried powder |
Molecular Mass : |
72kDa as determined by SDS-PAGE reducing conditions. |
Endotoxin : |
<1.0EU per 1µg (determined by the LAL method). |
Purity : |
>95% |
Characteristic : |
The isoelectric point is 6. |
Applications : |
SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : |
200µg/mL |
Storage buffer : |
PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. |
Reconstitution : |
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |