| Description : |
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
C-His |
| Form : |
Solution |
| Molecular Mass : |
71 kDa |
| Protein Length : |
654 |
| AA Sequence : |
MKLSLVAAMLLLLSAARAEEEDKKE DVGTVVGIDLGTTYSCVGVFKNGRV EIIANDQGNRITPSYVAFTPEGERL IGDAAKNQLTSNPENTVFDAKRLIG RTWNDPSVQQDIKFLPFKVVEKKTK PYIQVDIGGGQTKTFAPEEISAMVL TKMKETAEAYLGKKVTHAVVTVPAY FNDAQRQATKDAGTIAGLNVMRIIN EPTAAAIAYGLDKREGEKNILVFDL GGGTFDVSLLTIDNGVFEVVATNGD THLGGEDFDQRVMEHFIKLYKKKTG KDVRKDNRAVQKLRREVEKAKRALS SQHQARIEIESFYEGEDFSETLTRA KFEELNMDLFRSTMKPVQKVLEDSD LKKSDIDEIVLVGGSTRIPKIQQLV KEFFNGKEPSRGINPDEAVAYGAAV QAGVLSGDQDTGDLVLLDVCPLTLG IETVGGVMTKLIPRNTVVPTKKSQI FSTASDNQPTVTIKVYEGERPLTKD NHLLGTFDLTGIPPAPRGVPQIEVT FEIDVNGILRVTAEDKGTGNKNKIT ITNDQNRLTPEEIERMVNDAEKFAE EDKKLKERIDTRNELESYAYSLKNQ IGDKEKLGGKLSSEDKETMEKAVEE KIEWLESHQDADIEDFKAKKKELEE IVQPIISKLYGSAGPPPTGEEDTAE KDEL |
| Purity : |
> 90% |
| Applications : |
WB |
| Stability : |
This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use. |
| Storage : |
4°C |
| Concentration : |
1 mg/mL |
| Storage Buffer : |
Tris-HCl (pH 8). Sterile-filtered colorless solution. |
| Reconstitution : |
Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance. |
