Recombinant Human HTRA1 protein, His & GST-tagged

• Cat.No.: HTRA1-1859H
• Product Overview: Recombinant Human HTRA1 aa. (Gly204~Leu364) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 47kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly204~Leu364
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 6.6

Gene Information

• Gene Name: HTRA1 HtrA serine peptidase 1 [ Homo sapiens (human) ]
• Official Symbol: HTRA1
• Synonyms: HTRA1; HtrA serine peptidase 1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2; serine protease HTRA1; IGFBP5-protease; high-temperature requirement A serine peptidase 1; protease, serine, 11 (IGF binding)
• Gene ID: 5654
• mRNA Refseq: NM_002775.4
• Protein Refseq: NP_002766.1
• UniProt ID: Q92743