Recombinant Human HTT protein, MBP-His-tagged

• Cat.No.: HTT-1536H
• Product Overview: Recombinant Human HTT protein(Met1-Glu82), fused with His and MBP tag, was expressed in E.coli .

Specification

• Source: E.coli
• Species: Human
• Tag: MBP-His
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Molecular Mass: This protein carries a MBP tag at the N-terminus and a polyhistidine tag at the C-terminusThe protein has a calculated MW of 52.7 kDa. The protein migrates as 60-63 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Met1-Glu82
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HTT huntingtin [ Homo sapiens ]
• Official Symbol: HTT
• Synonyms: HTT; huntingtin; HD, huntingtin (Huntington disease); IT15; huntington disease protein; HD
• Gene ID: 3064
• mRNA Refseq: NM_002111
• Protein Refseq: NP_002102
• MIM: 613004
• UniProt ID: P42858