Recombinant Human IBA57 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: IBA57-6341H
• Product Overview: C1orf69 MS Standard C13 and N15-labeled recombinant protein (NP_001010867) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene localizes to the mitochondrion and is part of the iron-sulfur cluster assembly pathway. The encoded protein functions late in the biosynthesis of mitochondrial 4Fe-4S proteins. Defects in this gene have been associated with autosomal recessive spastic paraplegia-74 and with multiple mitochondrial dysfunctions syndrome-3. Two transcript variants encoding different isoforms have been found for this gene. The smaller isoform is not likely to be localized to the mitochondrion since it lacks the amino-terminal transit peptide.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 38 kDa
• AA Sequence: MATAALLRGATPGRGGPVWRWRLRA APRCRLAHSSCSPGGDPTAGAAWAC FRLDGRTLLRVRGPDAAPFLLGLLT NELPLPSPAAAGAPPAARAGYAHFL NVQGRTLYDVILYGLQEHSEVSGFL LECDSSVQGALQKHLALYRIRRKVT VEPHPELRVWAVLPSSPEACGAASL QERAGAAAILIRDPRTARMGWRLLT QDEGPALVPGGRLGDLWDYHQHRYL QGVPEGVRDLPPGVALPLESNLAFM NGVSFTKGCYIGQELTARTHHMGVI RKRLFPVRFLDPLPTSGITPGATVL TASGQTVGKFRAGQGNVGLALLWSE KIKGPLHIRASEGAQVALAASVPDW WPTVSKTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: IBA57 iron-sulfur cluster assembly factor IBA57 [ Homo sapiens (human) ]
• Official Symbol: IBA57
• Synonyms: IBA57; IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae); C1orf69, chromosome 1 open reading frame 69; putative transferase CAF17, mitochondrial; FLJ12734; iron sulfur cluster assembly factor for biotin synthase and aconitase like mitochondrial proteins; with a mass of 57kDa; iron-sulfur cluster assembly factor homolog; putative transferase C1orf69, mitochondrial; iron-sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins, with a mass of 57kDa; C1orf69; FLJ13849
• Gene ID: 200205
• mRNA Refseq: NM_001010867
• Protein Refseq: NP_001010867
• MIM: 615316
• UniProt ID: Q5T440