Recombinant Human IBSP Protein, His-tagged

• Cat.No.: IBSP-11H
• Product Overview: IBSP produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 309 amino acids (17-317aa) and having a molecular mass of 34.5 kDa (Migrates at 70-100 kDa on SDS-PAGE under reducing conditions).
  IBSP is expressed with a 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Specification

• Description: The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: Sterile Filtered colorless solution
• Molecular Mass: 34.5kDa
• Protein Length: 17-317
• AA Sequence: FSMKNLHRRVKIEDSEENGVFKYRP RYYLYKHAYFYPHLKRFPVQGSSDS SEENGDDSSEEEEEEEETSNEGENN EESNEDEDSEAENTTLSATTLGYGE DATPGTGYTGLAAIQLPKKAGDITN KATKEKESDEEEEEEEEGNENEESE AEVDENEQGINGTSTNSTEAENGNG SSGGDNGEEGEEESVTGANAEDTTE TGRQGKGTSKTTTSPNGGFEPTTPP QVYRTTSPPFGKTTTVEYEGEYEYT GANEYDNGYEIYESENGEPRGDNYR AYEDEYSYFKGQGYDGYDGQNYYHH QLEHHHHHH.
• Purity: > 90% SDS-PAGE
• Usage: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Storage: Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.
  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  Avoid multiple freeze-thaw cycles.
• Concentration: 0.5 mg/mL
• Storage Buffer: Phosphate Buffered Saline (pH 7.4), 10% glycerol and 0.1mM PMSF.
• Shipping: Shipped with Ice Packs

Gene Information

• Gene Name: IBSP integrin binding sialoprotein [ Homo sapiens (human) ]
• Official Symbol: IBSP
• Synonyms: IBSP; integrin binding sialoprotein; BSP; BNSP; SP-II; BSP-II; bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein
• Gene ID: 3381
• mRNA Refseq: NM_004967
• Protein Refseq: NP_004958
• MIM: 147563
• UniProt ID: P21815