Active Recombinant Human ICOSLG protein, hFc-tagged, Low Endotoxin

• Cat.No.: ICOSLG-425H
• Product Overview: Low Endotoxin Active Recombinant Human ICOSLG protein(O75144-1)(Asp19-Ser258) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 19-258 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human ICOS, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ICOSLG inducible T-cell co-stimulator ligand [ Homo sapiens ]
• Official Symbol: ICOSLG
• Synonyms: ICOSLG; inducible T-cell co-stimulator ligand; ICOSL; ICOS ligand; B7 homolog 2; B7 homologue 2; B7 H2; B7 related protein 1; B7H2; B7RP 1; B7RP1; CD275; GL50; ICOS L; KIAA0653; B7-like protein Gl50; B7-related protein 1; transmembrane protein B7-H2 ICOS ligand; B7-H2; LICOS; B7RP-1; ICOS-L
• Gene ID: 23308
• mRNA Refseq: NM_015259
• Protein Refseq: NP_056074
• MIM: 605717
• UniProt ID: O75144