Recombinant Human IDO1, His-tagged

• Cat.No.: IDO1-3909H
• Product Overview: Recombinant Human IDO1 (Ala2-Gly403) with an N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Indoleamine 2,3-dioxygenase(IDO) is a hemecontaining intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan toN-formylkynurenine. This degradation is the first and ratelimiting step of the L-kynurenine pathway. IDO is widely expressed in dendritic cells, macrophages,microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-γ, IFN-α,IFN-β, and IL-10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments.Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia.
• Molecular Mass: The recombinant human IDO1 predicts a molecular mass of 46 kDa. It migrates as 42 kDa band in SDS-PAGE under reducing conditions.
• Predict N-terminal: Met
• Structure: Monomer
• Formulation: Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Activity: Measured by its ability to oxidize L-tryptophan to N-formylkynurenine. The specific activity is >500 pmol/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
• Stability &Storage: Use a manual defrost freezer and avoid repeated freeze/thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 month, -70 °C under sterile conditions after opening.
• OfficialSymbol: IDO1

Gene Information

• Gene Name: IDO1 indoleamine 2,3-dioxygenase 1 [ Homo sapiens ]
• Synonyms: IDO1; indoleamine 2,3-dioxygenase 1; IDO; INDO; IDO-1; indoleamine 2,3-dioxygenase 1; EC 1.13.11.52; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; Indoleamine-pyrrole 2
• Gene ID: 3620
• mRNA Refseq: NM_002164
• Protein Refseq: NP_002155
• MIM: 147435
• UniProt ID: P14902
• Chromosome Location: 8p12-p11
• Pathway: African trypanosomiasis; Metabolism of amino acids and derivatives; Tryptophan catabolism; tryptophan degradation XI (mammalian, via kynurenine)
• Function: electron carrier activity; heme binding; indoleamine 2,3-dioxygenase activity; metal ion binding; oxygen binding; tryptophan 2,3-dioxygenase activity; amino acid binding