| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
24-188 aa |
| Form : |
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Bio-activity : |
Interferon alpha-2 (IFNα2) is a cytokine belonging to the family of typeI IFNs. Inaddition to their antiviral activity, IFNα2 also inhibit the proliferation of cellsandregulate the activation of the immune system. Type I IFNs exert potent antitumoractivity by several mechanisms such as inhibition of the proliferationof cancercells, activation of the immune system which can eliminate tumor cells and increasing the antitumor activity of other antitumoral agents. Besides, Interferon Alpha/Beta Receptor 2 (IFNa/bR2) has been identified as an interactor of IFNα2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IFNα2 and recombinant human IFNa/bR2. Briefly, IFNα2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uLwerethen transferred to IFNa/bR2-coated microtiter wells and incubated for 2hat 37°C. Wells were washed with PBST and incubated for 1h with anti-IFNα2pAb, thenaspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IFNα2and IFNa/bR2 was in a dose dependent manner. |
| Molecular Mass : |
23.0kDa |
| Purity : |
> 95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
