Recombinant Human IFNA5 protein(Leu22-Glu189), hFc-tagged

Cat.No. : IFNA5-30H
Product Overview : Recombinant Human IFNα G (NP_002160.1) (Leu 22-Glu 189) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.
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Source : HEK293
Species : Human
Tag : N-hFc
Protein length : Leu22-Glu189
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is 0.16-0.8 ng/mL.
Molecular Mass : The recombinant human IFNα G/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 405 amino acids with a predicted molecular mass of 46.3 kDa. As a result of glycosylation, rh IFNα G/Fc monomer migrates as an approximately 48-50 kDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : IFNA5 interferon, alpha 5 [ Homo sapiens ]
Official Symbol : IFNA5
Synonyms : IFNA5; interferon, alpha 5; interferon alpha-5; IFN alphaG; leIF G; IFN-alpha-5; interferon alpha-G; interferon alpha-61; INFA5; IFN-alphaG;
Gene ID : 3442
mRNA Refseq : NM_002169
Protein Refseq : NP_002160
MIM : 147565
UniProt ID : P01569

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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