Recombinant Human IFNGR2, Fc Chimera

• Cat.No.: IFNGR2-23H
• Product Overview: Recombinant Human IFNGR2 encoding the signal peptide and extracellular domain of human interferon gamma receptor 2 (aa 1-247) chain was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: IFNGR2-23H
• Description: Interferon-gamma (IFN-gamma) is a pleotropic cytokine expressed predominantly by naïve and activated CD8+ and TH1 CD4+ T cells, and natural killer (NK) cells and, as such, promotes both innate and adaptive immune responses.
• Source: Human 293 cells.
• Amino Acid Sequence: SQLPAPQHPKIRLYNAEQVLSWEPV ALSNSTRPVVYRVQFKYTDSKWFTA DIMSIGVNCTQITATECDFTAASPS AGFPMDFNVTLRLRAELGALHSAWV TMPWFQHYRNVTVGPPENIEVTPGE GSLIIRFSSPFDIADTSTAFFCYYV HYWEKGGIQQVKGPFRSNSISLDNL KPSRVYCLQVQAQLLWNKSNIFRVG HLSNISCYETMADASTELQQGSSNT KVDKKVEPKSCDKTHTCPPCPAPEL LGGPSVFLFPPKPKDTLMISRTPEV TCVVVDVSHEDPEVKFNWYVDGVEV HNAKTKPREEQYNSTYRVVSVLTVL HQDWLNGKEYKCKVSNKALPAPIEK TISKAKGQPREPQVYTLPPSRDELT KNQVSLTCLVKGFYPSDIAVEWESN GQPENNYKTTPPVLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALHN HYTQKSLSLSPGK.
• Molecular Mass: IFN-gamma R2 – Fc Chimera migrates as a broad band between 65 and 75 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IFN-gamma R2 - Fc Chimera that has a predicted molecular mass of 52 kDa.
• pI: IFN-gamma R2 – Fc Chimera separates into a number of glycoforms with a pI between 5.5 and 8 on 2D PAGE due to post-translational modifications, in particular glycosylation.
• % Carbohydrate: Purified IFN-gamma R2 – Fc Chimera consists of 20-30% carbohydrate by weight.
• Glycosylation: IFN-gamma R2 – Fc Chimera has N- and possibly O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Protein length: 1-247

Gene Information

• Gene Name: IFNGR2 interferon gamma receptor 2 (interferon gamma transducer 1) [ Homo sapiens ]
• Synonyms: IFNGR2; interferon gamma receptor 2 (interferon gamma transducer 1); AF-1; IFGR2; IFNGT1; Interferon-gamma receptor accessory factor 1; interferon gamma receptor accessory factor-1; Interferon-gamma transducer 1; interferon gamma receptor beta chain; interferon-gamma receptor beta chain precursor
• Gene ID: 3460
• mRNA Refseq: NM_005534
• Protein Refseq: NP_005525
• UniProt ID: P38484
• Chromosome Location: 21q22.1
• MIM: 147569
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity
• Function: interferon-gamma receptor activity; receptor activity