"IgG1Fc" Related Products


Recombinant Human IgG1Fc Protein, His & Avi-tagged, Biotinylated

Cat.No.: IgG1Fc-11H
Product Overview: Biotinylated polyhistidine tagged human IgG1 Fc (IG1-H82E2) is expressed from human HEK293 cells. It contains AA Pro 100 - Lys 330 (Accession # P01857(C103S)).
Source: HEK293
Species: Human
Tag: His & Avi
Predicted N Terminal: Ala
Form: Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA against immobilized Recombinant Protein A (0.2 μg/well). The linear range is 0.8-12.5 ng/mL.
Molecular Mass: This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 28.8 kDa. The protein migrates as 36 kDa on a SDS-PAGE gel under reducing condition due to glycosylation and 60-66 kDa under non-reducing condition.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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