Recombinant Human IgG1Fc Protein, His-tagged

• Cat.No.: IgG1Fc-13H
• Product Overview: Human IgG Fc, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 99-Lys 330 (Accession # P01857-1).

Specification

• Description: Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: The protein has a calculated MW of 26.9 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Glu 99-Lys 330
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) [ Homo sapiens (human) ]
• Official Symbol: IgG1Fc
• Synonyms: IgG1
• Gene ID: 3500
• MIM: 147100
• UniProt ID: P01857