Recombinant Human IGHG4 Protein (SPR verified)
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|Product Overview :||Recombinant Human IGHG4 Protein (SPR verified) (Glu99-Lys327) was expressed in human 293 cells (HEK293).|
|Description :||Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).|
|Predicted N Terminal :||Glu99|
|Form :||Tris with Glycine, Arginine and NaCl, pH7.5|
|Molecular Mass :||The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).|
|Protein length :||Glu99-Lys327|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.|
|SDS-PAGE of IGHG4-8282H :||
Human IgG4 Fc, Tag Free (SPR verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-SPR of IGHG4-8282H :||
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free (SPR verified) with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K) (QC tested).
|Gene Name :||IGHG4|
|Official Symbol :||IGHG4|
|Gene ID :||3503|
|UniProt ID :||P01861|
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