Recombinant Human IGHG4 Protein (SPR verified) IGHG4-8282H

Recombinant Human IGHG4 Protein (SPR verified)

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Recombinant Human IGHG4 Protein (SPR verified)

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Cat.No. : IGHG4-8282H
Product Overview : Recombinant Human IGHG4 Protein (SPR verified) (Glu99-Lys327) was expressed in human 293 cells (HEK293).
Description : Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
Source : HEK293
Species : Human
Predicted N Terminal : Glu99
Form : Tris with Glycine, Arginine and NaCl, pH7.5
Molecular Mass : The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : Glu99-Lys327
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of IGHG4-8282H :

Human IgG4 Fc, Tag Free (SPR verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR of IGHG4-8282H :

Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free (SPR verified) with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K) (QC tested).

Gene Name : IGHG4
Official Symbol : IGHG4
Synonyms : IGHG4
Gene ID : 3503
MIM : 147130
UniProt ID : P01861

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