Recombinant Human IGHG4 Protein (SPR verified)

• Cat.No.: IGHG4-8282H
• Product Overview: Recombinant Human IGHG4 Protein (SPR verified) (Glu99-Lys327) was expressed in human 293 cells (HEK293).

Specification

• Description: Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
• Source: HEK293
• Species: Human
• Predicted N Terminal: Glu99
• Form: Tris with Glycine, Arginine and NaCl, pH7.5
• Molecular Mass: The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).
• Protein length: Glu99-Lys327
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IGHG4
• Official Symbol: IGHG4
• Synonyms: IGHG4
• Gene ID: 3503
• MIM: 147130
• UniProt ID: P01861