Recombinant Human IGHG4 Protein (SPR verified) IGHG4-8282H

Recombinant Human IGHG4 Protein (SPR verified)


Home / Products / Recombinant Proteins / Recombinant Human IGHG4 Protein (SPR verified)

Recombinant Human IGHG4 Protein (SPR verified)

IGHG4 Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : IGHG4-8282H
Product Overview : Recombinant Human IGHG4 Protein (SPR verified) (Glu99-Lys327) was expressed in human 293 cells (HEK293).
Description : Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
Source : HEK293
Species : Human
Predicted N Terminal : Glu99
Form : Tris with Glycine, Arginine and NaCl, pH7.5
Molecular Mass : The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : Glu99-Lys327
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of IGHG4-8282H :

Human IgG4 Fc, Tag Free (SPR verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR of IGHG4-8282H :

Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free (SPR verified) with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K) (QC tested).

Gene Name : IGHG4
Official Symbol : IGHG4
Synonyms : IGHG4
Gene ID : 3503
MIM : 147130
UniProt ID : P01861

Online Inquiry

  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy