| Description : |
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
57.3 kDa |
| AA Sequence : |
MDADEGQDMSQVSGKESPPVSDTPD EGDEPMPIPEDLSTTSGGQQSSKSD RVVASNVKVETQSDEENGRACEMNG EECAEDLRMLDASGEKMNGSHRDQG SSALSGVGGIRLPNGKLKCDICGII CIGPNVLMVHKRSHTGERPFQCNQC GASFTQKGNLLRHIKLHSGEKPFKC HLCNYACRRRDALTGHLRTHSVGKP HKCGYCGRSYKQRSSLEEHKERCHN YLESMGLPGTLYPVIKEETNHSEMA EDLCKIGSERSLVLDRLASNVAKRK SSMPQKFLGDKGLSDTPYDSSASYE KENEMMKSHVMDQAINNAINYLGAE SLRPLVQTPPGGSEVVPVISPMYQL HKPLAEGTPRSNHSAQDSAVENLLL LSKAKLVPSEREASPSNSCQDSTDT ESNNEEQRSGLIYLTNHIAPHARNG LSLKEEHRAYDLLRAASENSQDALR VVSTSGEQMKVYKCEHCRVLFLDHV MYTIHMGCHGFRDPFECNMCGYHSQ DRYEFSSHITRGEHRFHMSTRTRPL EQKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
