Recombinant Human IL11RA Protein, GST-tagged

• Cat.No.: IL11RA-561H
• Product Overview: Recombinant Human IL11RA Protien(NP_001136256)(184-361 aa), fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Protein length: 184-361 aa
• AA Sequence: GAEFWSQYRINVTEVNPLGASTRLL DVSLQSILRPDPPQGLRVESVPGYP RRLRASWTYPASWPCQPHFLLKFRL QYRPAQHPAWSTVEPAGLEEVITDA VAGLPHAVRVSARDFLDAGTWSTWS PEAWGTPSTGTIPKEIPAWGQLHTQ PEVEPQVDSPAPPRPSLQPHPRLLD HRD
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

Gene Information

• Gene Name: IL11RA interleukin 11 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL11RA
• Synonyms: IL11RA; interleukin 11 receptor, alpha; interleukin-11 receptor subunit alpha; IL-11RA; IL-11R subunit alpha; IL-11 receptor subunit alpha; interleukin-11 receptor alpha chain; CRSDA; MGC2146
• Gene ID: 3590
• mRNA Refseq: NM_001142784
• Protein Refseq: NP_001136256
• MIM: 600939
• UniProt ID: Q14626

Reviews

02/15/2021

High affinity binding.

09/05/2020

Stable in solution.

02/20/2017

Effective in hematopoiesis studies.

Q&As

Can you explain how phosphoproteomics and mass spectrometry shed light on IL11RA-mediated signaling cascades and post-translational modifications?

Phosphoproteomics and mass spectrometry reveal IL11RA-related signaling pathways.

How are flow cytometry and fluorescence-activated cell sorting (FACS) employed to analyze IL11RA expression on different cell populations?

Flow cytometry and FACS analyze IL11RA expression on different cell types.

Could you elaborate on how X-ray crystallography and cryo-electron microscopy contribute to understanding the three-dimensional structure of IL11RA?

X-ray crystallography and cryo-EM reveal the three-dimensional structure of IL11RA.

What insights have gene expression profiling and RNA sequencing provided regarding IL11RA-related molecular pathways and networks?

Gene expression profiling and RNA sequencing unveil IL11RA-associated molecular networks.

How do techniques like co-immunoprecipitation and pull-down assays validate IL11RA's interactions with IL11 and other signaling partners?

Co-immunoprecipitation and pull-down assays confirm IL11RA interactions with IL11 and other partners.

What role does site-directed mutagenesis play in identifying IL11RA residues critical for IL11 binding and downstream signaling activation?

Site-directed mutagenesis identifies IL11RA residues crucial for binding and signaling.

How are animal models like transgenic mice and tissue-specific knockouts utilized to study the in vivo roles of IL11RA in development and disease?

Transgenic mice and knockouts study IL11RA's roles in development and disease.

Can you explain the experimental strategies used to characterize IL11RA isoforms and their differential roles in IL11 signaling?

Experimental strategies characterize IL11RA isoforms and their distinct roles in IL11 signaling.

How are biophysical techniques such as isothermal titration calorimetry and surface plasmon resonance used to determine IL11RA's binding affinities and kinetics?

Isothermal titration calorimetry and surface plasmon resonance measure IL11RA binding properties.

What methods like CRISPR-Cas9 knockout and RNA interference help elucidate the functional significance of IL11RA in specific biological contexts?

CRISPR-Cas9 knockout and RNA interference reveal IL11RA's functions in specific contexts.