Active Recombinant Human IL12 protein

• Cat.No.: IL12-47H
• Product Overview: Active Recombinant Human IL12 protein(P29459)(23-328 aa & 23-219 aa) was expressed in Sf 21 insect cell.

Specification

• Species: Human
• Source: Sf 21 insect cell
• Tag: Non
• Protein Length: 23-328 aa & 23-219 aa
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.2.
• Bio-activity: Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1 × 10^4 units/μg, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544).
• Molecular Mass: Apparent molecular mass of 60 kDa in SDS-PAGE under non-reducing conditions, 41 kDa and 29 kDa under reducing conditions, a disulfide-linked heterodimer consisting of the 306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits.
• Endotoxin: Less than 1.0 EU/µg of rHuIL-12 as determined by LAL method.
• Purity: > 97 % by SDS-PAGE.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.