| Cat.No. : |
IL35-1039H |
| Product Overview : |
Recombinant Human IL12A & IL27B Heterotrimer Protein (NP_000873.2)(Met1-Ser219) & (NP_005746.2)(Met1-Lys229) was produced by HEK293 Cells expression system. The IL12A protein was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The IL27B protein was fused with a flag tag at the C-terminus, constructed the plasmid 1. The two plasmids were co-expressed and the human IL27B&IL12A heterodimer was purified. |
| Description : |
IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His-Flag |
| Predicted N Terminal : |
Arg 21 & Arg 23 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Molecular Mass : |
The recombinant heterodimer of human IL27B&IL12A comprises 425 (217+208) amino acids and has a calculated molecular mass of 48.3(24.3+24) KDa. |
| Protein length : |
Met1-Ser219 & Met1-Lys229 |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
> 85 % as determined by SDS-PAGE. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70 centigrade. |
| Storage : |
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| Shipping : |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
