Recombinant Human IL12A & IL27B protein, Fc-Flag&Fc-His-tagged

• Cat.No.: IL12A & IL27B-2191H
• Product Overview: Recombinant Human IL12A & IL27B protein(NP_005746.2)(Met1-Ser219 & Met1-Lys229), fused with Fc-Flag&Fc-His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&Flag&His
• Protein Length: Met1-Ser219 & Met1-Lys229
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human IL12A&IL27B comprises 905 (446+459) amino acids and has a calculated molecular mass of 102.1 (50.6+ 51.5) KDa. The apparent molecular mass of the recombinant human IL12A&IL27B is approximately 66 KDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.