Recombinant Human IL12B Protein, Fc-tagged

Cat.No. : IL12B-503H
Product Overview : Recombinant human IL12B protein with a Fc tag
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Description : This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Species : Human
Tag : Fc
Form : Lyophilized
Protein Length : 328
AA Sequence : MCHQQLVISWFSLVFLASPLVAIWE LKKDVYVVELDWYPDAPGEMVVLTC DTPEEDGITWTLDQSSEVLGSGKTL TIQVKEFGDAGQYTCHKGGEVLSHS LLLLHKKEDGIWSTDILKDQKEPKN KTFLRCEAKNYSGRFTCWWLTTIST DLTFSVKSSRGSSDPQGVTCGAATL SAERVRGDNKEYEYSVECQEDSACP AAEESLPIEVMVDAVHKLKYENYTS SFFIRDIIKPDPPKNLQLKPLKNSR QVEVSWEYPDTWSTPHSYFSLTFCV QVQGKSKREKKDRVFTDKTSATVIC RKNASISVRAQDRYYSSSWSEWASV PCS
Purity : > 98%
Applications : WB; ELISA; FACS; FC
Stability : This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
Storage : At -20 centigrade.
Storage Buffer : PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
Reconstitution : Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
Gene Name : IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens (human) ]
Official Symbol : IL12B
Synonyms : IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B;
Gene ID : 3593
mRNA Refseq : NM_002187
Protein Refseq : NP_002178
MIM : 161561
UniProt ID : P29460

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
04/08/2020

    Good for cytokine assays.

    01/06/2020

      Effective in Th2 cell differentiation.

      01/12/2019

        High yield in expression.

        Q&As (10)

        Ask a question
        Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation? 05/12/2021

        ChIP-seq identifies regulatory elements controlling IL12B gene expression.

        Can you elaborate on the use of reporter assays to monitor IL12B promoter activity? 05/02/2021

        Reporter assays monitor IL12B promoter activity under varying conditions.

        What methods evaluate IL12B's response to different pathogenic stimuli in primary cells? 10/30/2020

        Primary cell stimulation assays gauge IL12B response to pathogens.

        How does siRNA knockdown of IL12B elucidate its role in immune cell activation? 05/25/2020

        siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

        How does recombinant protein expression and purification aid in IL12B functional studies? 04/30/2020

        Recombinant protein expression enables functional IL12B studies in vitro.

        How does co-immunoprecipitation validate IL12B's association with signaling partners? 04/12/2018

        Co-IP validates IL12B's binding to specific signaling partners.

        What techniques are employed to analyze the impact of IL12B genetic variants on protein function? 12/03/2017

        Genetic variant analysis reveals impacts on IL12B protein function.

        How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets? 10/31/2017

        Flow cytometry profiles IL12B expression across diverse immune cell types.

        What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation? 09/29/2017

        Cytokine secretion assays measure IL12B's response kinetics to stimuli.

        What are the methods to assess IL12B's protein-protein interactions in a cellular context? 08/22/2017

        Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.

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