Active Recombinant Human IL12B protein, Fc-tagged

• Cat.No.: IL12B-310H
• Product Overview: Active Recombinant Human IL12B protein(NP_002178.2) (Met 1-Ser 328) was expressed in Human Cells, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: Human Cells
• Tag: Fc
• Protein Length: 1-328 aa
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human IL12RB1 at 20 μg/ml (100 μl/well) can bind human IL12B with a linear ranger of 2.56-64 ng/ml.
• Molecular Mass: The recombinant human IL12B/Fc is a disulfide-linked homodimer. The reduced monomer consists of 544 amino acids and has a predicted molecular mass of 61.4 kDa. As a result of glycosylation, the rhIL12B/Fc monomer migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Official Symbol: IL12B
• Synonyms: IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• UniProt ID: P29460