Recombinant Human IL12B Protein, His-tagged
| Cat.No. : | IL12B-2290H |
| Product Overview : | Recombinant Human IL12B Protein (Val30-Ser320) with a N-His tag was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
| Description : | This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. |
| Source : | E. coli |
| Species : | Human |
| Tag : | N-His |
| Form : | Freeze-dried powder |
| Molecular Mass : | Predicted Molecular Mass: 36.62 kDa Accurate Molecular Mass: 44 kDa |
| Protein length : | Val30-Ser320 |
| Endotoxin : | <1.0 EU per 1µg (determined by the LAL method). |
| Purity : | > 97% |
| Applications : | Positive Control; Immunogen; SDS-PAGE; WB. |
| Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
| Storage Buffer : | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
| Gene Name : | IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens (human) ] |
| Official Symbol : | IL12B |
| Synonyms : | IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B; |
| Gene ID : | 3593 |
| mRNA Refseq : | NM_002187 |
| Protein Refseq : | NP_002178 |
| MIM : | 161561 |
| UniProt ID : | P29460 |
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| ◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (10)
Ask a question
Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?
05/12/2021
ChIP-seq identifies regulatory elements controlling IL12B gene expression.
Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?
05/02/2021
Reporter assays monitor IL12B promoter activity under varying conditions.
What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?
10/30/2020
Primary cell stimulation assays gauge IL12B response to pathogens.
How does siRNA knockdown of IL12B elucidate its role in immune cell activation?
05/25/2020
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
How does recombinant protein expression and purification aid in IL12B functional studies?
04/30/2020
Recombinant protein expression enables functional IL12B studies in vitro.
How does co-immunoprecipitation validate IL12B's association with signaling partners?
04/12/2018
Co-IP validates IL12B's binding to specific signaling partners.
What techniques are employed to analyze the impact of IL12B genetic variants on protein function?
12/03/2017
Genetic variant analysis reveals impacts on IL12B protein function.
How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?
10/31/2017
Flow cytometry profiles IL12B expression across diverse immune cell types.
What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?
09/29/2017
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
What are the methods to assess IL12B's protein-protein interactions in a cellular context?
08/22/2017
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Customer Reviews (3)
Write a reviewReviews
04/08/2020
Good for cytokine assays. -
01/06/2020
Effective in Th2 cell differentiation. -
01/12/2019
High yield in expression. -
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