Active Recombinant Human IL12RB1 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL12RB1-051H
• Product Overview: Biotinylated Recombinant Human IL12RB1(Cys24-Glu540) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells .

Specification

• Description: IL12RB1 (Interleukin-12 receptor subunit beta-1; IL‑12 R beta 1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).
• Source: CHO cells
• Species: Human
• Tag: Fc/Avi
• Predicted N Terminal: Cys24
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human IL12 is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL12RB1 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.
• Molecular Mass: 95-115 kDa, under reducing conditions
• Protein length: Cys24-Glu540
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.

Gene Information

• Gene Name: IL12RB1 interleukin 12 receptor, beta 1 [ Homo sapiens ]
• Official Symbol: IL12RB1
• Synonyms: IL12RB1; interleukin 12 receptor, beta 1; IL12RB; interleukin-12 receptor subunit beta-1; CD212; IL-12RB1; IL-12R-beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; interleukin-12 receptor beta-1 chain; IL-12R-BETA1; MGC34454
• Gene ID: 3594
• mRNA Refseq: NM_005535
• Protein Refseq: NP_005526
• MIM: 601604
• UniProt ID: P42701