| Cat.No. : |
IL12RB1-73H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human IL12RB1 (Cys24-Glu540) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293). |
| Description : |
The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
human IgG1 Fc |
| Predicted N Terminal : |
Cys24 |
| Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 83.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
Cys24-Glu540 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by reduced SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of IL12RB1-73H : |
Human IL-12 R beta 1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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| Bioactivity-ELISA of IL12RB1-73H : |
Immobilized Human IL-12B, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag with a linear range of 5-78 ng/mL (QC tested).
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
