Recombinant Human IL13RA1 protein, hFc-tagged

• Cat.No.: IL13RA1-063H
• Product Overview: Human IL-13 R alpha 1 Protein is expressed from mammalian with a hFc tag at the C-terminal. It contains Ala27 - Thr343.

Specification

• Description: Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Supplied as 0. 22um filtered solution in PBS (pH 7. 4).
• Molecular Mass: The protein has a predicted MW of 63. 4 kDa. Due to glycosylation, the protein migrates to 78-90 KDa based on Bis-Tris PAGE result.
• Protein length: It contains Ala27 - Thr343.
• Endotoxin: Less than 0. 05 EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IL13RA1 interleukin 13 receptor, alpha 1 [ Homo sapiens ]
• Official Symbol: IL13RA1
• Gene ID: 3597
• mRNA Refseq: NM_001560
• Protein Refseq: NP_001551
• MIM: 300119
• UniProt ID: P78552

Reviews

01/10/2022

High solubility.

01/17/2019

Potent in anti-allergic assays.

04/26/2018

Suitable for ELISA.

Q&As

What techniques determine IL13RA1 expression levels in different cell types?

Techniques quantify IL13RA1 expression across diverse cell types.

What experimental strategies discern IL13RA1 isoforms and their functions?

Strategies distinguish IL13RA1 isoforms and their roles.

Could you elaborate on the use of RNA-seq in profiling IL13RA1-regulated gene expression?

RNA-seq profiles IL13RA1's influence on gene expression.

What are the advantages of using CRISPR/Cas9 to knockout IL13RA1 expression?

CRISPR/Cas9 knockout clarifies IL13RA1's functional impact.

How do siRNA knockdown experiments validate IL13RA1's involvement in specific pathways?

siRNA knockdown validates IL13RA1's pathway involvement.

Can you explain the role of co-immunoprecipitation in identifying IL13RA1 interaction partners?

Co-immunoprecipitation identifies IL13RA1 interactors.

How does immunofluorescence microscopy visualize IL13RA1 localization within cells?

Immunofluorescence microscopy visualizes intracellular IL13RA1 localization.

How does surface plasmon resonance (SPR) elucidate IL13RA1 binding kinetics?

SPR elucidates IL13RA1 binding kinetics with precision.

Can you describe how surface biotinylation studies reveal IL13RA1 membrane localization?

Surface biotinylation shows IL13RA1 membrane distribution.

How do fluorescence-based assays assess IL13RA1 internalization upon ligand binding?

Fluorescence assays monitor IL13RA1 internalization upon ligand binding.