Recombinant Human IL15RA protein, His-Avi-tagged, Biotinylated

• Cat.No.: IL15RA-273HB
• Product Overview: Biotinylated Recombinant Human IL15R alpha Protein is expressed from mammalian with a His and Avi tag at the C-terminal. It contains Asp 21 - Glu 335.

Specification

• Description: Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a predicted MW of 21.3 kDa.Due to glycosylation, the protein migrates to 50- 70KDa based on Bis-Tris PAGE result.
• Protein length: It contains Asp 21 - Glu 335.
• Endotoxin: Less than 0.1EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IL15RA interleukin 15 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL15RA
• Synonyms: IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1
• Gene ID: 3601
• mRNA Refseq: NM_001243539
• Protein Refseq: NP_001230468
• MIM: 601070
• UniProt ID: Q13261

Reviews

10/29/2020

Effective in T-cell assays.

03/10/2018

High purity levels.

05/28/2017

Consistent receptor binding.

Q&As

How does immunoprecipitation validate IL15RA's association with IL2RB?

Immunoprecipitation validates IL15RA's binding to IL2RB in cells.

What are the applications of CRISPR/Cas9 in studying IL15RA knockout models?

CRISPR/Cas9 technology aids in generating IL15RA knockout models.

Could you explain the approaches to investigate IL15RA's role in autoimmune diseases?

Approaches unravel IL15RA's implication in autoimmune disease mechanisms.

What experimental strategies differentiate IL15RA's role in innate and adaptive immunity?

Methods discern IL15RA's distinct roles in innate and adaptive immunity.

What techniques characterize IL15RA isoform expression in tissues?

RT-PCR and Western blotting assess IL15RA isoform expression patterns.

How is IL15RA's binding affinity to IL15 measured in vitro?

Binding affinity of IL15RA to IL15 quantified through surface plasmon resonance.

What methods explore IL15RA's involvement in immune cell proliferation?

Proliferation assays investigate IL15RA's contribution to immune cell growth.

How is IL15RA's interaction with JAK/STAT pathways analyzed?

IL15RA's interaction with JAK/STAT pathways probed using immunoblotting.

How do siRNA knockdown experiments reveal IL15RA's role in signaling?

siRNA knockdown studies unveil IL15RA's impact on downstream signaling.

Can you detail the use of FRET assays to study IL15RA dimerization?

FRET assays employed to elucidate IL15RA dimerization kinetics.