Recombinant Human IL17C 293 Cell Lysate

• Cat.No.: IL17C-5243HCL

Specification

• Description: Antigen standard for interleukin 17C (IL17C) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: IL17C interleukin 17C [ Homo sapiens ]
• Official Symbol: IL17C
• Synonyms: IL17C; interleukin 17C; interleukin-17C; CX2; IL 17C; IL 21; MGC126884; MGC138401; cytokine CX2; IL-21; IL-17C
• Gene ID: 27189
• mRNA Refseq: NM_013278
• Protein Refseq: NP_037410
• MIM: 604628
• UniProt ID: Q9P0M4
• Chromosome Location: 16q24
• Function: cytokine activity

Reviews

11/26/2021

High purity levels.

03/26/2021

Potent in anti-fungal assays.

06/07/2020

Suitable for receptor binding studies.

Q&As

How is the involvement of IL17C in autoimmune diseases elucidated using experimental models and patient samples?

Animal models, patient biopsies, and genetic association studies reveal IL17C's role in autoimmune diseases.

Can you explain the experimental designs used to study the crosstalk between IL17C and other cytokines in inflammatory responses?

Combination cytokine stimulation assays and signaling pathway inhibitors elucidate IL17C's crosstalk.

How is the function of IL17C in promoting tumor growth and progression investigated in cancer models?

Xenograft models and tumor growth measurements uncover IL17C's contribution to cancer progression.

How are the effects of IL17C modulation on specific immune cell populations characterized using flow cytometry and cell sorting?

Flow cytometry analysis with cell-specific markers characterizes IL17C's impact on immune cell subsets.

What molecular techniques are utilized to validate the therapeutic potential of targeting IL17C in inflammatory disorders?

Neutralizing antibodies and RNA interference validate IL17C's potential as a therapeutic target.

What approaches are taken to assess the role of IL17C in mucosal immunity and host defense against infections?

Infection models, bacterial challenges, and mucosal sampling assess IL17C's role in host defense.

Could you elaborate on the methods used to study IL17C's contribution to tissue-specific immune cell recruitment?

Chemotaxis assays and confocal microscopy study IL17C-mediated immune cell recruitment.

What strategies are used to explore the impact of IL17C on epithelial barrier integrity and inflammation?

Organoid culture systems and cytokine measurements explore IL17C's impact on epithelial barrier function.

What experimental techniques are employed to investigate the binding partners of IL17C and its downstream signaling pathways?

Co-immunoprecipitation and mass spectrometry identify IL17C interactors and signaling molecules.

How do researchers differentiate the functional roles of IL17C from other IL17 family members in immune responses?

Comparative functional assays and gene knockout models distinguish IL17C's unique roles.