Recombinant Human IL18RAP Protein, His-tagged

• Cat.No.: IL18RAP-157H
• Product Overview: Recombinant Human IL18RAP Protein with His tag was expressed in E. coli.

Specification

• Description: Interleukin-18 (IL-18) has been identified as a molecule that induces IFN-γ production and enhances NK cell cytotoxicity. IL-18 receptor (IL-18R) is a type I membrane protein present in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta and heart, and absent from brain, skeletal muscle, pancreas and kidney. IL-18R is present in Hodgkin’s disease cell lines, and does not bind IL-1α or IL-1β. The association of IL-18 to IL-18R leads to activation of NFκB. At present two subunits of IL-18R have been characterized: IL-18Rα and IL-18Rβ. IL-18Rα has been described as the ligand-binding chain and IL-18Rβ as the signal-transduction chain.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~43 kDa
• AA Sequence: MFNISGCSTKKLLWTYSTRSEEEFV LFCDLPEPQKSHFCHRNRLSPKQVP EHLPFMGSNDLSDVQWYQQPSNGDP LEDIRKSYPHIIQDKCTLHFLTPGV NNSGSYICRPKMIKSPYDVACCVKM ILEVKPQTNASCEYSASHKQDLLLG STGSISCPSLSCQSDAQSPAVTWYK NGKLLSVERSNRIVVDEVYDYHQGT YVCDYTQSDTVSSWTVRAVVQVRTI VGDTKLKPDILDPVEDTLEVELGKP LTISCKARFGFERVFNPVIKWYIKD SDLEWEVSVPEAKSIKSTLKDEIIE RNIILEKVTQRDLRRKFVCFVQNSI GNTTQSVQLKEKR
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: IL18RAP interleukin 18 receptor accessory protein [ Homo sapiens (human) ]
• Official Symbol: IL18RAP
• Synonyms: IL18RAP; interleukin 18 receptor accessory protein; interleukin-18 receptor accessory protein; AcPL; CD218b; IL-1R7; IL-1R-7; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta; IL-18 receptor beta; interleukin-1 receptor 7; interleukin-18 receptor beta; IL-18 receptor accessory protein; CD218 antigen-like family member B; ACPL; IL18RB; CDw218b; MGC120589; MGC120590
• Gene ID: 8807
• mRNA Refseq: NM_003853
• Protein Refseq: NP_003844
• MIM: 604509
• UniProt ID: O95256

Reviews

02/09/2021

Effective in signaling studies.

11/17/2020

High specificity.

09/16/2018

Reliable in co-immunoprecipitation.

Q&As

What experimental methods are commonly employed to study the expression patterns and regulation of IL18RAP across different cell types and conditions?

RT-qPCR, immunohistochemistry, and single-cell RNA sequencing characterize IL18RAP expression levels and regulation in diverse cellular contexts.

What techniques are utilized to investigate the role of IL18RAP in immune responses, inflammation, and diseases, and how do researchers assess its potential as a therapeutic target?

Animal models, patient samples, and therapeutic interventions elucidate IL18RAP's involvement in immune-related disorders and its potential as a therapeutic target.

Could you provide insights into the experimental approaches used to explore the functional effects of IL18RAP isoforms and their specific contributions to IL18 signaling?

Isoform-specific knockdown, mutagenesis, and functional assays delineate IL18RAP isoforms' distinct roles in modulating IL18-mediated responses.

How are co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and biophysical assays applied to characterize the interactions between IL18RAP, IL18, and IL18R1?

Co-immunoprecipitation assays, FRET studies, and biophysical techniques characterize IL18RAP's interactions with IL18 and IL18R1 partners.

What experimental methods are used to elucidate the effects of post-translational modifications, such as glycosylation, on IL18RAP's structure and function?

Mass spectrometry, mutagenesis, and functional assays investigate the impact of post-translational modifications on IL18RAP's conformation and function.

How do researchers experimentally determine the binding affinity and kinetics of IL18RAP to IL18 and IL18R1, considering its pivotal role in forming the IL18 receptor complex?

Surface plasmon resonance, bio-layer interferometry, and surface plasmon-enhanced fluorescence assays quantify IL18RAP's binding kinetics to IL18 and IL18R1.

Can you explain the use of knockdown, knockout, and gene editing techniques to uncover the physiological importance of IL18RAP in mediating IL18 signaling?

siRNA knockdown, CRISPR/Cas9-mediated knockout, and transgenic models unravel IL18RAP's physiological roles and contributions to IL18 signaling.

How are advanced imaging techniques, such as single-molecule imaging and super-resolution microscopy, utilized to visualize the dynamic assembly of the IL18 receptor complex involving IL18RAP?

Single-molecule imaging, super-resolution microscopy, and live-cell imaging visualize the dynamic assembly of IL18 receptor complexes involving IL18RAP.

Can you elaborate on the strategies used to investigate the structural features of IL18RAP's interaction interfaces with IL18 and IL18R1, and how these interfaces contribute to receptor activation?

X-ray crystallography, NMR spectroscopy, and computational modeling reveal IL18RAP's structural interactions with IL18 and IL18R1 domains.

How do researchers dissect the intracellular signaling pathways triggered by IL18RAP upon binding to IL18 and its collaboration with IL18R1?

Phosphoprotein analysis, pathway-specific inhibitors, and functional assays dissect IL18RAP's intracellular signaling cascades and its crosstalk with IL18R1.