Recombinant Human IL1F9 293 Cell Lysate
- Gene Information
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|Description :||Antigen standard for interleukin 1 family, member 9 (IL1F9) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.|
|Source :||HEK 293 cells|
|Components :||This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).|
|Size :||0.1 mg|
|Storage Instruction :||Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.|
|Applications :||ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.|
|Gene Name :||IL36G interleukin 36, gamma [ Homo sapiens ]|
|Official Symbol :||IL36G|
|Synonyms :||IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2; interleukin-36 gamma; IL-1 epsilon; IL-1-epsilon; IL-1(EPSILON); interleukin-1 epsilon; IL-1 related protein 2; IL-1-related protein 2; interleukin-1 homolog 1; interleukin-1 family member 9; interleukin 1 family, member 9; interleukin 1-related protein 2|
|Gene ID :||56300|
|mRNA Refseq :||NM_019618|
|Protein Refseq :||NP_062564|
|UniProt ID :||Q9NZH8|
|Chromosome Location :||2q12-q21|
|Function :||cytokine activity; interleukin-1 receptor binding|
|◆ Recombinant Protein|
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|IL36G-13H||Recombinant Human IL36G Protein, His-tagged||+Inquiry|
|Il36g-176M||Active Recombinant Mouse Il36g Protein||+Inquiry|
|IL36G-198H||Recombinant Active Human IL36G Protein, His-tagged(C-ter)||+Inquiry|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (6)Ask a question
It is currently mainly used in the treatment of skin inflammatory diseases, and the therapeutic potential of other inflammatory diseases needs further study.
It is mainly used in the research of the mechanism of skin inflammation and the development of new drugs.
The production of IL36G usually adopts genetic engineering technology, its genes are introduced into the expression host, and pure is obtained through fermentation, purification and other steps.
IL36G can be targeted to inhibit inflammation by directly acting on inflammation-related signaling pathways, which may be more effective than other drugs.
IL36G activates inflammation-related signaling pathways by binding to its receptors, promoting the activation of immune cells and the release of inflammatory factors.
The side effects of IL36G may include skin irritation, allergic reactions, etc. The specific side effects can vary from individual to individual.
Customer Reviews (3)Write a review
During the production process, it is necessary to ensure the correct folding and structure of IL-36G similar to the natural form to ensure its function and biological activity. -
IL-36B is detected under different temperature and time conditions to observe its change in thermal stability. When producing IL-36G, it is necessary to ensure its high purity, that is, the protein does not contain other impurities, to ensure the best function and effect. -
Production of IL-36G is usually carried out by cellular expression systems, such as bacterial or mammalian cells, to produce the protein on a large scale. -
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