Recombinant Human IL1RAP Protein, hFc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: IL1RAP-627HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human IL1RAP (Ser21-Glu359) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Interleukin-1 receptor accessory protein (IL1RAP or IL-1RAcP) is also known as Interleukin-1 receptor 3 (IL-1R-3 or IL-1R3), C3orf13, which belongs to the interleukin-1 receptor family. IL1RAP contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RAP is detected in liver, skin, placenta, thymus and lung. IL-1R3/IL1RAP is coreceptor with IL1R1, associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 65.7 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ser21-Glu359
• N-terminal Sequence Analysis: Ser 21
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: IL1RAP
• Official Symbol: IL1RAP
• Synonyms: IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein; C3orf13; IL 1RAcP; IL1R3; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta; IL-1RAcP; FLJ37788
• Gene ID: 3556
• mRNA Refseq: NM_001167928
• Protein Refseq: NP_001161400
• MIM: 602626
• UniProt ID: Q9NPH3

Reviews

05/25/2021

High solubility.

07/22/2019

Good for intracellular staining.

05/13/2018

Effective in apoptosis assays.

Q&As

How can single-cell RNA sequencing and proteomics uncover IL1RAP's interactions with immune receptors within complex networks?

Single-cell RNA sequencing and proteomics unveil IL1RAP's interactions in networks.

Can genome-wide mapping and functional assays reveal the epigenetic regulation and context-specific expression of the IL1RAP gene?

Genome mapping and assays unveil IL1RAP's epigenetic control.

How can we unravel IL1RAP's varied distribution across cell types and tissues, clarifying its functional versatility?

Innovative methods clarify IL1RAP's distribution diversity, revealing its functional adaptability.

How do advanced imaging and genetic knockout models shed light on IL1RAP's role in immune cell dynamics and functions?

Advanced imaging and genetic models illuminate IL1RAP's immune role.

What insights do computational simulations and in vitro experiments provide into IL1RAP's interaction kinetics and stability?

Simulations and experiments reveal IL1RAP's interaction details.

Do engineered cellular models clarify IL1RAP's mechanisms in inflammasome activation and cytokine release?

Engineered cell models shed light on IL1RAP's role in inflammation.

Can structural biology techniques elucidate IL1RAP's conformational changes and ligand interactions in inflammation?

Structural techniques provide insights into IL1RAP's ligand interactions.

What strategies unveil the influence of post-translational modifications on IL1RAP's localization and signaling pathways?

Strategies uncover post-translational effects on IL1RAP's signaling.

How do organ-on-chip technologies mimic tissue microenvironments to explore IL1RAP's impact on signaling pathways?

Organ-on-chip tech explores IL1RAP's impact on pathways.

What innovative methods decode IL1RAP isoforms in different cells, revealing their impact on cellular responses?

Novel techniques decode IL1RAP isoforms, exposing their influence on cell responses.