Active Recombinant Human IL1RN protein, His-tagged
Cat.No. : | IL1RN-495H |
Product Overview : | Active Recombinant Human IL1RN protein(P18510)(26~177 aa), fused with N-terminal His tag, was expressed in HEK293. |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
Protein Length : | 26-177 aa |
Form : | Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
Bio-activity : | IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R), the same receptor that binds interleukin 1 (IL-1), preventing IL-1 from sending a signal to that cell. Besides, Interleukin 1 Alpha (IL-1a) has been identified as an interactor of IL-1RA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-1RA and recombinant human IL-1a. Briefly, IL1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to IL-1a-coated microtiter wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-IL-1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1a was shown, and this effect was in a dose dependent manner. |
Molecular Mass : | 18.7kDa |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | > 95% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | IL1RN interleukin 1 receptor antagonist [ Homo sapiens ] |
Official Symbol | IL1RN |
Synonyms | IL1RN; interleukin 1 receptor antagonist; interleukin-1 receptor antagonist protein; ICIL 1RA; IL 1RN; IL1F3; IL1RA; interleukin 1 receptor antagonist protein; intracellular interleukin 1 receptor antagonist; IRAP; MGC10430; IL1 inhibitor; IL1RN (IL1F3); type II interleukin-1 receptor antagonist; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); DIRA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA; |
Gene ID | 3557 |
mRNA Refseq | NM_000577 |
Protein Refseq | NP_000568 |
MIM | 147679 |
UniProt ID | P18510 |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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