Recombinant Human IL2-Inducible T-Cell Kinase, GST-tagged

• Cat.No.: ITK-5438H
• Product Overview: Recombinanthuman full length ITK, GST-tagged. Activated in vitro viaauto-phosphorylation.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: ITK is a member of the TEC family of non-receptor tyrosinekinases. ITK is expressed in T-cells and is important for T-cell developmentand activation through the antigen receptor. ITK require prior activation ofLck, Zap-70 and PI3-kinase for efficient activation and shares major substrateswith both Lck and Zap-70. ITK knockout mice show multiple effects on T celldevelopment, cytokine production and T-helper cell differentiation. T cellsthat lack or express mutant versions of ITK show impaired TCR-induced actinpolymerization, cell polarization and regulation of the signaling eventsinvolved in cytoskeletal reorganization.
• Form: Liquidin 50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
• Molecular Weight: 99.9kDa
• Storage: Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: ITK
• Full Length: Full L.

Gene Information

• Gene Name: ITKIL2-inducible T-cell kinase [ Homo sapiens ]
• Synonyms: ITK; IL2-inducible T-cell kinase; EMT; LYK; PSCTK2; MGC126257;MGC126258; tyrosine-protein kinase LYK; homolog of mouse T-cell itk/tsk;tyrosine-protein kinase ITK/TSK; EC 2.7.10.2; Kinase EMT
• Gene ID: 3702
• mRNA Refseq: NM_005546
• Protein Refseq: NP_005537
• MIM: 186973
• UniProt ID: Q08881
• Chromosome Location: 5q31-q32
• Pathway: Chemokine signaling pathway;Leukocyte transendothelial migration; T cell receptor signaling pathway; AdaptiveImmune System; B Cell Receptor Signaling Pathway; Class I PI3K signaling events;Fc-epsilon receptor I signaling in mast cells
• Function: ATP binding; metal ion binding;non-membrane spanning protein tyrosine kinase activity; nucleotide binding;protein binding; transferase activity; zinc ion binding