Recombinant Human IL20 293 Cell Lysate

• Cat.No.: IL20-5233HCL

Specification

• Description: Antigen standard for interleukin 20 (IL20) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: IL20 interleukin 20 [ Homo sapiens ]
• Official Symbol: IL20
• Synonyms: IL20; interleukin 20; interleukin-20; IL 20; IL10D; ZCYTO10; cytokine Zcyto10; four alpha helix cytokine; IL-20; MGC96907
• Gene ID: 50604
• mRNA Refseq: NM_018724
• Protein Refseq: NP_061194
• MIM: 605619
• UniProt ID: Q9NYY1
• Chromosome Location: 1q32
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; interleukin-20 receptor binding; receptor binding

Reviews

04/14/2023

The reliability and reproducibility of the IL20 Protein's performance make it a go-to option for WB experiments.

12/13/2022

The IL20 protein is of exceptional quality and will undoubtedly meet my experimental needs with utmost reliability and accuracy.

06/25/2021

By utilizing the IL20 protein in my experiments, I can trust that I am working with a product of the highest caliber, backed by a company dedicated to delivering exceptional quality and support.

Q&As

How does IL-20 influence tissue repair and regeneration?

IL-20 has been implicated in tissue repair processes, and harnessing its properties may have implications for regenerative medicine.

How is IL-20 protein clinically applied in treating autoimmune diseases?

IL-20 is being explored as a potential therapeutic target in autoimmune diseases due to its involvement in immune system regulation.

In what ways does IL-20 contribute to inflammatory responses?

IL-20 is known to promote inflammation, and understanding its mechanisms may lead to targeted anti-inflammatory therapies.

Are there any ongoing clinical trials involving IL-20 protein?

Yes, there are several clinical trials exploring the safety and efficacy of IL-20 inhibitors in various disease contexts.

Can IL-20 be a target for personalized medicine in autoimmune conditions?

Tailoring treatments based on individual IL-20 levels and responses is a potential avenue for personalized medicine.