Recombinant Human IL21R protein, Fc-tagged IL21R-245H

Recombinant Human IL21R protein, Fc-tagged

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Recombinant Human IL21R protein, Fc-tagged

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Cat.No. : IL21R-245H
Product Overview : Recombinant Human IL21R protein (Cys20-Pro236) was fused to human IgG1 Fc tag at the C-terminus and expressed in human 293 cells (HEK293).
Description : Interleukin-21 receptor (IL21R) is also known as CD antigen CD360, Novel interleukin receptor (NILR), is a receptor for interleukin-21 and belongs to the type I cytokine receptor family or type 4 subfamily. IL21R contains two fibronectin type-III domains. IL21R is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen. IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Cys20
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 51.6 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing (R) condition, and 120-130 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : Cys20-Pro236
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of IL21R-245H :

Human IL-21 R, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA of IL21R-245H :

Immobilized Biotinylated Human IL-21, Fc,Avitag at 2 μg/mL (100 μL/well) can bind Human IL-21 R, Fc Tag with a linear range of 4-63 ng/mL (QC tested).

Gene Name : IL21R
Official Symbol : IL21R
Synonyms : NILR; CD360; IMD56
Gene ID : 50615
mRNA Refseq : NM_021798.4
Protein Refseq : NP_068570.1
MIM : 605383
UniProt ID : Q9HBE5

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