|Product Overview:||Recombinant Human IL21R(Cys20-Pro236) fused with His tag at C-terminal was expressed in HEK293.|
|Description:||Interleukin-21 Receptor (IL-21 R )is a type I transmembrane glycoprotein within the class I cytokine receptor family. IL-21 R associates with the common gamma chain ( gamma c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 285 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation. Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes. Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture. B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses. IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production, control of viral infection and tumor growth by CD8+ T cells, development of regulatory T cells, IL-23 responsiveness of encephalitogenic Th17 cells, but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway. IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL).|
|Predicted N Terminal:||Cys20|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured by its ability to inhibit IL-21-dependent proliferation of N1186 human T cells Parrish-Novak, J. et al. (2000) Nature 408:57. The ED50 for this effect is typically 0.6-3.6 µg/mL in the presence of 100 ng/mL of recombinant human IL-21.|
|Molecular Mass:||Predicted Molecular Mass: 26 kDa
SDS-PAGE: 45-53 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 500 μg/mL in PBS.|
|Gene Name:||IL21R interleukin 21 receptor [ Homo sapiens ]|
|Synonyms:||IL21R; interleukin 21 receptor; interleukin-21 receptor; CD360; IL-21R; IL-21 receptor; novel interleukin receptor; NILR; MGC10967;|
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