Recombinant Human IL27RA cell lysate
| Cat.No. : | IL27RA-2909HCL |
| Product Overview : | Human IL27Ra / TCCR / WSX1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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| Source : | Human cells |
| Species : | Human |
| Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
| Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
| Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
| Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
| Gene Name : | IL27RA interleukin 27 receptor, alpha [ Homo sapiens ] |
| Official Symbol : | IL27RA |
| Synonyms : | IL27RA; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; CRL1; IL 27R; T cell cytokine receptor type 1; TCCR; WSX 1; WSX1; zcytor1; IL-27RA; IL-27R-alpha; IL-27R subunit alpha; cytokine receptor-like 1; class I cytokine receptor; IL-27 receptor subunit alpha; T-cell cytokine receptor type 1; type I T-cell cytokine receptor; IL27R; |
| Gene ID : | 9466 |
| mRNA Refseq : | NM_004843 |
| Protein Refseq : | NP_004834 |
| MIM : | 605350 |
| UniProt ID : | Q6UWB1 |
| Chromosome Location : | 19p13.11 |
| Pathway : | IL27-mediated signaling events, organism-specific biosystem; |
| Function : | interleukin-27 receptor activity; receptor activity; transmembrane signaling receptor activity; |
Products Types
| ◆ Recombinant Protein | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionIn general, Il27ra should be administered by subcutaneous injection. For specific use, follow your doctor's advice and read the drug label carefully.
Il27ra regulates the function of immune cells by binding to its receptor, inhibiting inflammatory response and enhancing antiviral ability.
It is mainly used in clinical treatment of immune-related diseases, such as tumors, autoimmune diseases and so on.
It can be used in conjunction with other medications in certain cases, but be sure to inform your doctor about the medications you are using to avoid the possibility of drug interactions.
The use of Il27ra in children is not yet clear, please follow the guidance of your doctor.
No significant side effects of recombinant Il27ra have been found, but common adverse reactions such as local reactions and anaphylaxis may occur.
Customer Reviews (3)
Write a reviewTechniques such as ELISA or flow cytometry can be used to detect IL-27RA concentrations in body fluids or cells. -
Molecular structure of IL-27RA protein was analyzed by crystallography or NMR, and the binding mode and interaction mechanism with IL-27 were revealed. -
The regulation of IL-27RA on cell signal transduction pathway and changes in cell function were evaluated by cell experiments. -
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