Recombinant Human IL27RA cell lysate

Cat.No. : IL27RA-2909HCL
Product Overview : Human IL27Ra / TCCR / WSX1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL27RA interleukin 27 receptor, alpha [ Homo sapiens ]
Official Symbol : IL27RA
Synonyms : IL27RA; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; CRL1; IL 27R; T cell cytokine receptor type 1; TCCR; WSX 1; WSX1; zcytor1; IL-27RA; IL-27R-alpha; IL-27R subunit alpha; cytokine receptor-like 1; class I cytokine receptor; IL-27 receptor subunit alpha; T-cell cytokine receptor type 1; type I T-cell cytokine receptor; IL27R;
Gene ID : 9466
mRNA Refseq : NM_004843
Protein Refseq : NP_004834
MIM : 605350
UniProt ID : Q6UWB1
Chromosome Location : 19p13.11
Pathway : IL27-mediated signaling events, organism-specific biosystem;
Function : interleukin-27 receptor activity; receptor activity; transmembrane signaling receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the use of Il27ra? 07/02/2022

In general, Il27ra should be administered by subcutaneous injection. For specific use, follow your doctor's advice and read the drug label carefully.

What is the action mechanism of Il27ra? 02/10/2022

Il27ra regulates the function of immune cells by binding to its receptor, inhibiting inflammatory response and enhancing antiviral ability.

Does the clinical applications of Il27ra? 09/08/2020

It is mainly used in clinical treatment of immune-related diseases, such as tumors, autoimmune diseases and so on.

Can Il27ra be used with other drugs? 10/29/2019

It can be used in conjunction with other medications in certain cases, but be sure to inform your doctor about the medications you are using to avoid the possibility of drug interactions.

Can Il27ra be used in children? 09/27/2019

The use of Il27ra in children is not yet clear, please follow the guidance of your doctor.

What are the side effects of Il27ra? 08/19/2019

No significant side effects of recombinant Il27ra have been found, but common adverse reactions such as local reactions and anaphylaxis may occur.

Customer Reviews (3)

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03/19/2022

    Techniques such as ELISA or flow cytometry can be used to detect IL-27RA concentrations in body fluids or cells. -

    12/08/2021

      Molecular structure of IL-27RA protein was analyzed by crystallography or NMR, and the binding mode and interaction mechanism with IL-27 were revealed. -

      02/21/2020

        The regulation of IL-27RA on cell signal transduction pathway and changes in cell function were evaluated by cell experiments. -

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